Fluorometric Biosensing of the Total Amino of Green Tea Infusions Using an Automated
نویسنده
چکیده
An automated multi-channel continuous-flow analyzing system was constructed for the rapid measurement of both the tota} amino acid content and the L-gtutamate content of green tea infusions. i.-Glutamate in samples was oxidized and deaminated enzymaticatly in a channel -'ith a mini-reactor that "'as packed with i.-glutamate oxidase immebilized on glass beads, and the evolyed ammonium ions were measuTed by a fluorogenic reaction with o-phtha]aldeh)'de and fi-mercaptoethanol. The total amino acid content was measured in a channe] with another mini-reactor which was fi11ed with immobi]ized 1.-amino
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تاریخ انتشار 2018